Fig. 3.

Hsp27’s NTD plays an important role in chaperoning tau. a Representative tau aggregation curves in the presence of truncated Hsp27 constructs. Aggregation of 0N4R (left) or K18 (right) in the presence of listed chaperones at a 1:1 molar ratio (10 μM). Data points are the mean ± SEM of 3 technical replicates. RFU, relative fluorescence units. b Left, Negative-stain EM images of WT (left) and 3D (right) Hsp27. Images are representative of a minimum of 12 random fields. Scale bar is 200 nm. Right, SEC-MALS trace of Hsp27 WT and 3D (40 μM). c Intensity ratios of 50 μM ¹⁵N K18 in the presence of of 100 μM Hsp27 WT or 3D (2:1 molar ratio). d Representative 0N4R aggregation curves in the presence of Hsp27 WT or 3D at a 1:1 molar ratio (10 μM). For information on why the amplitude of the signal may be increased in the presence of chaperones, see Supplementary Figure 7C. Results are representative of independent experiments performed in quadruplicate (see Supplementary Figure 7A). Each individual experiment was performed in triplicate and error bars represent SEM