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. 2018 Nov 1;8:16220. doi: 10.1038/s41598-018-34474-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Villus and crypt harbor unique miRNA expression profile which is altered by CD98 overexpression. Relative fold change (2^−∆∆CT) of miRNAs detected in either villus or crypt of WT and CD98 Tg ileal IECs is shown. To determine if there was a regional variation in miRNA expression profile due to genotypic difference, Tg villi were compared with WT villi (a) and Tg crypt were compared with WT crypt (b). Of the 58 miRNAs identified from these comparisons, with the exclusion of miR-134-5p, -19 and -37, miRNA profile signature is unique to the villus or crypt. Y-axis is shown in Log₂ (Relative expression) scale.