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. 2018 Nov 1;8:16175. doi: 10.1038/s41598-018-34561-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Parallel evolution during serial passage in mosquito cells. (A) Venn diagram showing the repartition of the shared substitutions (in bold) during serial passage in mosquito cells. Each cell line is represented by an ellipse. Numbers in overlapping areas represent the number of substitutions found during passages in at least two different cell lines. Numbers in non-overlapping areas represent the number of substitutions found during passages in only one cell line (the number in bracket represents the number of shared substitutions shared in the same cell line). (B) Proportion of substitutions shared between viruses passaged in the same cell line. (C) Proportion of substitutions shared between viruses passaged in different cell lines (i.e. shared between at least two different cell lines). The maximum frequency was considered when one substitution was detected more than once.