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. 2018 Nov 1;8:16195. doi: 10.1038/s41598-018-34324-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Osteogenic differentiation associates with the upregulation of TRPM7 and MagT1. (A) hMSC were cultured in OM or CM for 3, 6, 10 and 14 days. Real-Time PCR was performed on RNA extracted from hMSC using primers designed on TRPM7 and MagT1 sequence. (B) Western blot was performed on extracts from hMSC cultured in OM or CM for 6 and 14 days using antibodies against TRPM7 or MagT1. Actin was used as a control of loading. A representative blot is shown and quantification is provided in the Supplementary information (Fig. S1A).