Figure 2.

Specific siRNAs silence TRPM7 or MagT1 in hMSC. (A) After exposure to siRNA, hMSC were cultured in CM for 3 days. Real-Time PCR was performed on RNA extracted from hMSC using primers designed on the sequence of TRPM7 and MagT1. The controls, indicated as -, were exposed to non-silencing, scrambled sequences. (B) Western blot was performed on extracts from hMSC after 3 days silencing. Antibodies against TRPM7 or MagT1 were used. Actin was used as a control of loading. A representative blot is shown and quantification is provided in the Supplementary information (Fig. S2A). (C) Total Mg was measured using the fluorescent chemosensor DCHQ5 as described.