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. 2018 Nov 1;8:16195. doi: 10.1038/s41598-018-34324-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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siRNAs targeting TRPM7 or MagT1 enhance osteogenic differentiation. (A) After exposure to siRNA, hMSC were cultured in CM or OM for 3 days. Real-Time PCR was performed on RNA extracted from hMSC using primers designed on RUNX2 and COL1A1 sequence. (B) ELISA for RUNX2 and collagen type 1 was conducted on extracts from hMSC cultured in CM or OM for 5 days. The controls, indicated as -, were exposed to non-silencing, scrambled sequences.