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. 2018 Nov 1;8:16195. doi: 10.1038/s41598-018-34324-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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siRNAs targeting MagT1 or TRPM7 induce the deposition of calcium. Alizarin Red S staining was performed after exposure to CM or OM for 14 days. Whole well image (upper left panel) and photographs taken at 10X magnification (lower panel) are shown. After acid extraction the absorbance was measured at 550 nm (upper right panel).