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. 2018 Nov 1;8:16219. doi: 10.1038/s41598-018-34504-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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13 consecutive serial sections of Ophirina amphinema showing ultrastructure of flagellar apparatus. PB, posterior basal body; PF, posterior flagellum. Black and white arrowheads indicate right root microtubule and singlet root microtubule, respectively. Double black arrowheads and double white arrowheads indicate left root microtubules and B-fibre, respectively. Stars indicate C-fibre. Scale bars = 200 nm. (a–d) Unilateral row of right root microtubules in ‘U-shape’ near their origin. Singlet root originates in front row of right root microtubules. Left root microtubules are underlain by C-fibre near their point of origination. (e–i). Singlet root microtubule runs posteriorly, joining row of left root microtubules. Right root splits into two parts. Inner part (2 microtubules) runs adjacent to assembly of singlet root and left root. Outer right root microtubules spread out and support right rim of groove. (j–m) Left root splits into two parts. Inner part of left root (MTs 1–3) splits again into two parts. Inner part of left root (MTs 1–2) runs together with singlet root, and in parallel with inner right root. MT 3 runs separately. Outer part (MTs 4–7) runs posteriorly, supporting left rim of groove.