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. 2018 Nov 1;8:16219. doi: 10.1038/s41598-018-34504-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transmission electron micrographs of Ophirina amphinema. AB, anterior basal body; PB, posterior basal body; PF, posterior flagellum; vg, ventral feeding groove; cf, composite fibre. Black arrowheads and arrows indicate right root microtubules and flagellar vane, respectively. White arrowheads and arrows indicate organizing centre for dorsal fan microtubules and dorsal fan microtubules, respectively. White double arrowheads indicate B-fibre. (a–e) Five consecutive serial sections showing origin of dorsal fan microtubules and apparent lack of distal fibres connecting basal bodies. Note that (c) is higher-magnification view of same cell and section shown in Fig. 2a. Scale bars = 200 nm. (f) Right root microtubules supporting right rim of groove, accompanied by composite fibre showing thin banded patterns. Position of posterior basal body and anterior outline of cell projected from nearby sections. Scale bar = 500 nm. (g) Thin fibrous structures supporting flagellar vane from inside are spaced at regular intervals and exist at right angle to flagellar axis. Scale bar = 200 nm. (h) Lateral section of flagellar vane shows tubular supporting structure. Scale bar = 400 nm.