Figure 1.

Enoxacin and bis-enoxacin, at a concentration of 50 µM, do not effect growth of 4T1 cancer cells or trigger apoptosis. (A) 4T1 murine breast cancer cells were plated at a density of 50 cells/250 µm². After the cells had adhered on Day 1 and every 24 H subsequently, the cells in 3 randomly selected 250 µm² squares (which was averaged) from at least 3 wells were counted by counters who were blinded to the treatment conditions. Data is presented +/− standard error. Asterisk indicates P < 0.05 determined by ANOVA and Student’s T test. (B) MTT assay was performed to examine proliferation of 4T1 cells. 2 × 10⁴ cells were plated in 96-well plates, treated with enoxacin, bis-enoxacin or vehicle as indicated and assayed after 2 days. The N for each condition as 8. Asterisk indicates P < 0.05 determined by ANOVA and Student’s T test. (C) 0.5 × 10⁴ cells/cm² were plated in 24-well plates and treated as indicated. Caspase-3 activity was assayed at the times indicated. N for each condition was 4. Asterisk indicates P < 0.05 determined by ANOVA and Student’s t test.