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. 2018 Oct 26;5:101. doi: 10.3389/fnut.2018.00101

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Copyright © 2018 Moran, Al-Rammahi, Batchelor, Bravo and Shirazi-Beechey.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of electric field stimulation on SGLT1 expression. Mouse intestinal loops were stimulated continuously for different periods of time, using frequency of 20 Hz, with 0.3 ms duration, 1 ms delay and 50 V in the absence/presence of TTX or remained unstimulated as described in Methods. (A) SGLT1 mRNA abundance as assessed by qPCR, in unstimulated tissue (Con), stimulated tissue (20 min), or tissue stimulated for 20 min in the presence of the 10 μM tetrodotoxin (TTX). (B) Abundance of SGLT1 protein, determined by western blot analysis, in BBMV isolated from mouse small intestinal tissue that was either stimulated for 20 or 30 min (20 and 30 min), in the presence of tetrodotoxin (TTX), or left unstimulated (Con). (C) Densitometric analysis of western blots normalized to β-actin protein abundance (n = 5–9). Data are expressed as means ± SEM. P-values are based on unpaired Student's t-tests. *P < 0.05; **P < 0.01.