FIGURE 3.

Community composition of single particles (3–4 individual particles pooled) and pooled lake particles according to MG-RAST metagenomics annotation. (A) The distribution of Archaea, Bacteria, Eukarya, and Viruses; (B) Bacterial families with a sequence frequency of more than 5% in at least one sample; (C) Eukaryotic families with a sequence frequency of more than 5% in at least one sample; (D) Virus families with a sequence frequency of more than 5% in at least one sample. The lower stacked bars in each panel represent individual samples (Replicate reactors R1–R4) while the upper ones the sequencing results of poled RNA from the pair of sample below. (E) Venn diagrams showing the taxonomic overlap between single particle samples (R1–R4) and pooled RNA extracts (R1 + R2 or R3 + R4) at the family level including Bacteria, Archaea, Eukaryotes, and Viruses. The diagrams do not account for abundance but only presence absence. The number of taxonomic entities in each group is shown in each circle of the diagram. The shading color of the text in the legend of the Venn diagram depicts the color of the individual sample circles. The color of squares represents the overlapping between the different samples.