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. 2018 Oct 26;9:2569. doi: 10.3389/fmicb.2018.02569

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Copyright © 2018 Bižić-Ionescu, Ionescu and Grossart.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A subset of functional modules involved in substrate degradation and utilization obtained from single particles (3–4 individual particles pooled) and pooled lake particles transcriptomes (A) The lower stacked bars represent individual samples while the upper ones the sequencing results of poled RNA from the pair of sample below. The shown modules are Level-3 MG-RAST subsystem hierarchy. Venn diagrams (B) show the overlap between all the recognized modules including the subset in panel A, between single particle samples (R1–R4) and pooled RNA extracts (R1 + R2 or R3 + R4). The number of modules identified in the transcriptome of each sample is given in each circle of the diagram. The shading color of the text in the legend of the Venn diagram depicts the color of the individual sample circles. The color of squares represents the overlapping between the different samples.