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. 2018 Nov 2;7(11):83. doi: 10.1038/s41389-018-0093-z

Fig. 4. Cell cycle arrest and apoptosis induced by compound BDF-1253.

Fig. 4

a Compound BDF-1253 induced cell cycle arrest at G0/G1 phase in RCC 786-O and A498 cells, compared to the untreated control. Data was shown in the figure as mean ± SD. Experiments were performed in n = 3 biological replicates. b Compound BDF-1253 induced apoptosis in RCC 786-O and A498 cells, in a dose-dependent manner. Representative results were shown, data was shown in figure as mean ± SD. Experiments were performed in n = 3 biological replicates. c Compound BDF-1253 decreased the mRNA expression levels of c-Myc, Bcl-2, and CDK6, in a dose-dependent manner. Gene-specific data were normalized to β2-microglobulin expression and shown as average expression relative to DMSO control. Standard deviations among 3 biological replicates were indicated as error bars. One-way ANOVA was used to compare compound treatment group to DMSO control. *p < 0.05; **p < 0.01; ***p < 0.001. d Compound BDF-1253 decreased the protein abundance of c-Myc, Bcl-2, and increased the protein abundance of Bax, cleaved-PARP, cleaved-Caspase3 in a dose-dependent manner