Fig. 5. RNA-Seq data analysis and KEGG pathways statistics.

a The p-values and names of the most over-represented KEGG pathways, calculated on the basis of all the differentially expressed genes after the treatment of compound BDF-1253. b Heatmap of all the differentially expressed genes from the cell cycle and p53 KEGG pathways, after the treatment of compound BDF-1253. c RT-PCR analysis validated the differentially expressed genes. Gene-specific data were normalized to β2-microglobulin expression and shown as average expression relative to DMSO control. Error bars indicated standard deviations among 3 technical replicates per experiment