Table 4.
Binding parameters, Kd (dissociation constant) (nM) and Rt (concentration of binding sites) (pmol/mg), for the Vip3 and Cry proteins estimated from the respective homologous competition assays with BBMV from S. frugiperda and H. armigera.
| Protein | Mean ± SE | |||||
|---|---|---|---|---|---|---|
| S. frugiperda | H. armigeraa | |||||
| K d | R t | K d1 | R t1 | K d2 | R t2 | |
| ARP150v02 | 103 ± 55 | 48 ± 39 | — | — | — | — |
| Vip3Aa | 17 ± 5 | 50 ± 11 | 54 ± 10 | 138 ± 23 | — | — |
| Cry1Fa | 0.30 ± 0.02 | 1.30 ± 0.05 | 0.045 ± 0.003 | 0.023 ± 0.014 | 6.0 ± 0.3 | 14.1 ± 0.2 |
| Cry2Ae | 3.4 ± 0.6 | 0.26 ± 0.04 | 1.19 ± 0.03 | 0.40 ± 0.03 | — | — |
aTwo binding sites were obtained for Cry1Fa in this insect species.