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. 2018 Oct 26;9:921. doi: 10.3389/fneur.2018.00921

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Copyright © 2018 Chu, Liu, Janowski, Bulte, Li, Pearl and Walczak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MRI and histological assessment post-BBBO. (a) T2-weighted, pre-Gd, and post-Gd images 3 days after BBBO showed no sign of brain damage. No Gd-CE could be observed in the brain, suggesting that the BBB was resealed. Fluorescent staining of the BBBO region with GFAP (b), Iba1 (c), and NeuN (d) revealed comparable intensity between the ipsilateral and the contralateral hemisphere (2 ROIs/hemisphere as represented in lower magnification, n = 4, mean ± SD) indicating no inflammation and no neuronal loss after BBBO.