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. 2018 Oct 26;9:1176. doi: 10.3389/fphar.2018.01176

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Copyright © 2018 Gianotti, Delpiano and Caci.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detached epithelial cells isolated from bronchus. Bronchus is placed in a 100-mm Petri-dish and epithelial cells are collected by flushing the bronchial lumen vigorously with physiological saline solution.