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Proliferative serum-free medium.
| Compound | Final concentration |
|---|---|
| LHC basal medium (Life Technology #12677-019) | 500 ml |
| RPMI 1640 | 500 ml |
| Stock 4 | 5 ml/L |
| Stock 11 | 0.5 ml/L |
| Calcium stock | 40.6 μM |
| Insulin stock | 0.4325 μM |
| Transferrin stock | 61.5 μM |
| EGF stock | 0.403 nM |
| P/E stock | 2.5 ml/L |
| RA/T3 stock | 0.05 ml/L |
| Trace element solution | 5 ml/L |
| HC stock | 0.1 μM |
| Bovine pituitary extract (BPE) (Gibco #13028-014) | 25 mg/L |
| Penicillin | 100 U/ml |
| Streptomycin | 100 μg/ml |
| L-glutamine | 2 mM |
