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Differentiation serum-supplemented medium.
| Compound | Final concentration |
|---|---|
| DMEM High glucose w/o L-glut | 500 ml |
| Ham’s F12 w/o L-glut | 500 ml |
| Insulin stock | 0.4325 μM |
| Transferrin stock | 61.5 μM |
| P/E stock | 2.5 ml/L |
| RA/T3 stock | 0.1 ml/L |
| HC stock | 0.2 μM |
| Bovine pituitary extract (BPE) (Gibco #13028-014) | 25 mg/L |
| New Zealand Serum | 20 ml/L |
| Penicillin | 100 U/ml |
| Streptomycin | 100 μg/ml |
| L-glutamine | 2 mM |
