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Differentiation serum-free medium.
| Compound | Final concentration |
|---|---|
| DMEM High glucose w/o L-glut | 500 ml |
| Ham’s F12 w/o L-glut | 500 ml |
| Insulin stock | 0.87 μM |
| Transferrin stock | 123 μM |
| EGF stock | 0.806 nM |
| Epinephrine stock | 2.73 μM |
| RA stock | 0.099 μM |
| T3 stock | 0.01 μM |
| HC stock | 1.4 μM |
| Bovine pituitary extract (BPE) (Gibco #13028-014) | 50 mg/L |
| BSA stock | 22.58 μM |
| Penicillin | 100 U/ml |
| Streptomycin | 100 μg/ml |
| L-glutamine | 2 mM |
