Table 1.
Species | Treatment | First significant impact on escape rate | First significant impact on survival rate at day 15 | First significant impact on insemination rate | First significant impact on full insemination rate |
---|---|---|---|---|---|
Aedes aegypti | Irradiation | 90 Gy | 90 Gy (0.819) | NA (0.951) | 90 Gy (0.840) |
Chilling | 8 °C | 0 °C (0.802) | NA (0.616) | 8 °C (0.532) | |
Compaction | 5 g | 5 g (0.776) | NA (0.879) | 15 g (0.812) | |
Aedes albopictus | Irradiation | 40 Gy | 40 Gy (0.918) | 40 Gy (0.790) | 20 Gy (0.859) |
The first values of the different treatments significantly impacting each male quality indicator are presented. The values in brackets correspond to the proportion of explained variance (r-square), used as a model quality indicator, based on a linear mixed-effect model where the response variable (survival, insemination and full insemination rates) is predicted using the escape rate as a fix effect and the repeats as random effects. All p-values of the predictions were below 0.001. Survival was quantified by removing and counting dead individuals from both control and experimental cages daily for a period of 15 days. Mating propensity was calculated by measuring the number of virgin females (n = 10) a single control or post stress treatment male could successfully inseminate during a period of 5 days. Females were scored as inseminated or fully inseminated if one or two or more spermatheca contained sperm respectively. NAs correspond to cases in which models did not converge, mostly because the insemination rate was 1 in some of the treatments.