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. 2018 Nov 1;9(11):1112. doi: 10.1038/s41419-018-1160-2

Fig. 5. Increasing mitochondrial apoptosis sensitivity establishes TRAIL/TL32711 response synergies in otherwise TRAIL resistant GBM cell lines.

Fig. 5

a Cells were treated with TRAIL (100 ng/ml) and/or TL32711 (1 µM) in the presence or absence of ABT-199 (10 μM) and zVAD-fmk (50 μM). Cell death was measured after 24 h by PI-based flow cytometry. Data represent mean ± SEM of three independent experiments. ***p < 0.001 (one-way ANOVA followed by Tukey post hoc test). b Caspase processing and substrate cleavage in non-responder cell lines treated alone or in combination with ABT-199 (5 μM), TRAIL (100 ng/ml) and TL32711 (100 nM). zVAD-fmk was used at 50 μM. Whole-cell lysates were analysed for the indicated proteins by western blotting. GAPDH served as loading control. Asterisks indicate unspecific bands. Representative results from three independent experiments are shown. c Non-responders are resistant to combinations of TRAIL or TL32711 with ABT-199 (100 ng/ml; 1 µM; 5 μM). Cell death was measured after 24 h by PI-based flow cytometry. Data represent mean ± SEM of three independent experiments