Table 1.
Antibody characteristics. VJ assignments, CDR3 sequences, % identity, and isotype for the antibody clones. IMGT/V-QUEST software was used to assign the germline reference for IGHV and IGLV and determine % identity to germline. ELISAs were done to determine reactivity and microneutralization assays were performed to determine neutralization activity at concentrations up to 100 µg per mL
| Antibody | V-Gene | J-Gene | CDR3 | % Identity | Isotype | V-Gene | J-Gene | CDR3 | % Identity | Isotype | Reactivity to PRVABC59 NS1 | Reactivity to MR766 NS1 | Neutralization activity (IC50) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AA12 | VH3-53 | JH3-02 | CARDRRGFDYW | 99% | IgG1 | VK1-39 | JK4-01 | CQQTYSTPLTF | 98% | Kappa | Yes | Yes | None detected |
| FC12 | VH3-53 | JH3-02 | CARGPVQLERRPLGAFDIW | 99% | IgG1 | VL3-1 | JL2-01 | CQAWDSSTVVF | 100% | Lambda | Yes | No | None detected |
| EB9 | VH3-53 | JH3-02 | CARWGGKRGGAFDIW | 100% | IgG1 | VK1-39 | JK2-01 | CQQSYSTPYTF | 98% | Kappa | Yes | Yes | None detected |
| GB5 | VH3-53 | JH3-01 | CARLIAAAGDYW | 99% | IgG1 | VK1-39 | JK1-01 | CQQSYSTPWTF | 98% | Kappa | Yes | Yes | None detected |