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. 2018 Nov 1;8:16231. doi: 10.1038/s41598-018-34485-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hydrogen peroxide (H₂O₂) increases intensity of endogenous chemiluminescence of A. thaliana seedlings in a dose-dependent manner. H₂O₂ was applied on germinating seeds after three days after a deposition on agar plate. There was always 60 s gap between injection of H₂O₂ and start of the measurement due to sample manipulation. Standard error of mean is calculated for n = 3 measurements.