FIGURE 4.
Effect of aromatic chemicals on S. scitamineum mating/filamentation or sporidial growth. (A) Microscopic imaging of hyphae formed after sexual mating, in WT or sskpp2Δ mutant, supplemented with cAMP (10 mM), tyrosol (20 μM) or tryptophol (20 μM), or without supplemented chemicals (CK) as a control. Images were taken 48 h post inoculation. Scale bar = 50 μm. Sporidial growth was assessed in the WT (solid line) or sskpp2Δ (dashed line) strain of MAT-1 (B) or MAT-2 (C) mating-type background. S. scitamineum sporidia was cultured in liquid YePS medium for 24 h before dilution to reach the concentration of 105 cells per ml. The diluted cells were cultured for a further 40 h in the absence (cube) or presence of tyrosol (20 μM; triangle) or tryptophol (20 μM; circle). The number of cells at each specific time point was acquired by measuring the OD absorption at 600 nm. The growth curve for each culture was prepared by plotting the logarithmic values of OD600 vs. incubation time. Mean ± S.E. are derived from three independent biological repeats, each of which contained three technical repeats. (D) Representative microscopic images of wild-type (WT) and the sskpp2Δ sporidia. n > 40 for each strain examined. Images were taken with Axio Observer Z1 microscope equipped with an sCMOS camera. Scale bar = 10 μm.