FIGURE 5.

Identification of potential target genes of Sskpp2-mediated MAPK signaling pathway. (A) qRT-PCR analysis with selected genes in the sskpp2Δ mutant in comparison to WT, under mating condition. Relative gene expression fold change was calculated with –ΔΔCt method (Livak and Schmittgen, 2001) with ACTIN as internal control. Mean ± S.E. are derived from two independent biological repeats, each of which contained three replica. ^∗ and ^∗∗ denote significant difference with p < 0.05 and p < 0.01, respectively. (B) Mating/filamentation of WT, sstyna1Δ and sstyna2Δ mutants was assessed on PDA solid medium. Photographs were taken 3 days post inoculation.