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. 2018 Oct 26;9:1475. doi: 10.3389/fphys.2018.01475

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Copyright © 2018 Trotta, Maisto, Alessio, Hermenean, D’Amico and Di Filippo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MC5R mRNA and protein levels. (A) RT-PCR analysis showed a significant up-regulation of MC5R in H9c2 cells exposed to high glucose (33 mM D-glucose) compared to cardiomyocytes exposed to normal glucose (5.5 mM D-glucose). (B) The significantMC5Rup-regulation in HG group was confirmed also by detection of MC5R protein levels by Western Blotting assay. Values are expressed as mean of 2^-ΔΔCt or D.U. ± S.E.M. of n = 9 values, obtained from the triplicates of three independent experiments. NG, normal glucose; HG, high glucose; D.U., Densitometric Units; ^∗P < 0,01 vs. NG.