Skip to main content
. 2018 Oct 1;8(11):1804–1819. doi: 10.1002/2211-5463.12518

Figure 1.

Figure 1

Interaction of TXNIP and cell cycle regulatory proteins in vitro and in vivo. (A) In vivo interaction analysis by immunoprecipitation. COS‐7 cells were transfected with plasmids as indicated. Immunoprecipitation followed by western blot analysis showed the interaction of TXNIP to each of p27kip1, JAB1, Cdk2, and cyclin E. (B) In vitro interaction analysis. GST‐p27kip1, GST–JAB1, GST–Cdk2, GST–cyclin E, and His–TXNIP were expressed in E. coli and affinity‐purified. Purified proteins were separated by SDS/PAGE, and the gels were stained with Coomassie Brilliant Blue R‐250. Interaction of purified His–TXNIP with each protein was analyzed, followed by western blot for TXNIP. IP, immunoprecipitation; WB, western blot.