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. 2018 Aug 16;46(19):10448–10459. doi: 10.1093/nar/gky737

Figure 3.

Figure 3.

RNA gel blot hybridizations demonstrating loss of dicistronic rpl16-rpl14 transcripts in maize and Arabidopsis pgr3 mutants. Seedling leaf RNA from maize (A) or Arabidopsis (B) plants of the indicated genotype was analyzed by RNA gel blot hybridization, using probes specific for the indicated regions. The maize atp1 mutant lacks the chloroplast ATP synthase (54) and was included to control for effects resulting from the loss of this complex. The first three lanes of the blots shown in (A) (WT, atp4 and atp1) were published previously (15) and are reproduced here with permission from: Zoschke, R., Watkins, K.P. and Barkan, A. (2013) A rapid ribosome profiling method elucidates chloroplast ribosome behavior in vivo. Plant Cell,25, 2265–2275; DOI:10.1105/tpc.113.111567, www.plantcell.org, Copyright American Society of Plant Biologists (2013). Arabidopsis svr7 is orthologous to maize atp4. The rpl16 and rpl14 blots in panel (B) came from the same gel, whereas the rps8 blot came from a different gel. The probe for rpl14 in Arabidopsis cross-hybridized with the 16S rRNA, as marked in (B).