Figure 7.
Effect of mutations in RbpA-BL on the kinetics of RPo formation and promoter escape. (A) RPo formation was monitored in single-round run-off transcription assays. (B) Promoter escape was monitored in single-round run-off transcription assays. Representative gels showing run-off [32P]-RNA products synthesized from the sigAP-WT promoter by σB-MtbRNAP in the presence of the indicated RbpA variants. (C) Quantification of the experiments shown in A and B (mean values ± SE of three experiments). All shown RNA products were used for quantification. (D) The half-times of RPo formation and promoter escape were determined from the plots shown in panel C.