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. 2018 May 31;31(12):1871–1880. doi: 10.5713/ajas.18.0041

Table 2.

Description of 20 microsatellite markers used in the current study

No. Primer Chromosomal location Primer sequence (5′→3′), Forward and reverse Annealing Temp. (°C) Allele size range (bp)
1 MCW0248 1 GTTGTTCAAAAGAAGATGCATG
TTGCATTAACTGGGCACTTTC
60 205–225
2 MCW0111 1 GCTCCATGTGAAGTGGTTTA
ATGTCCACTTGTCAATGATG
60 96–120
3 ADL0268 1 CTCCACCCCTCTCAGAACTA
CAACTTCCCATCTACCTACT
60 102–116
4 MCW0020 1 TCTTCTTTGACATGAATTGGCA
GCAAGGAAGATTTTGTACAAAATC
60 179–185
5 LEI0234 2 ATGCATCAGATTGGTATTCAA
CGTGGCTGTGAACAAATATG
60 216–364
6 MCW0206 2 ACATCTAGAATTGACTGTTCAC
CTTGACAGTGATGCATTAAATG
60 221–249
7 MCW0034 2 TGCACGCACTTACATACTTAGAGA
TGTCCTTCCAATTACATTCATGGG
60 212–246
8 MCW0103 3 AACTGCGTTGAGAGTGAATGC
TTTCCTAACTGGATGCTTCTG
64 266–270
9 LEI0166 3 CTCCTGCCCTTAGCTACGCA
TATCCCCTGGCTGGGAGTTT
60 354–370
10 MCW0295 4 ATCACTACAGAACACCCTCTC
TATGTATGCACGCAGATATCC
60 88–106
11 MCW0081 5 GTTGCTGAGAGCCTGGTGCAG
CCTGTATGTGGAATTACTTCTC
60 112–135
12 MCW0014 6 TATTGGCTCTAGGAACTGTC
GAAATGAAGGTAAGACTAGC
58 164–182
13 MCW0183 7 ATCCCAGTGTCGAGTATCCGA
TGAGATTTACTGGAGCCTGCC
58 296–326
14 ADL0278 8 CCAGCAGTCTACCTTCCTAT
TGTCATCCAAGAACAGTGTG
60 114–126
15 MCW0067 10 GCACTACTGTGTGCTGCAGTTT
GAGATGTAGTTGCCACATTCCGAC
60 176–186
16 MCW0104 13 TAGCACAACTCAAGCTGTGAG
AGACTTGCACAGCTGTGTACC
60 190–234
17 MCW0123 14 CCACTAGAAAAGAACATCCTC
GGCTGATGTAAGAAGGGATGA
60 76–100
18 MCW0330 17 TGGACCTCATCAGTCTGACAG
AATGTTCTCATAGAGTTCCTGC
60 256–300
19 MCW0165 23 CAGACATGCATGCCCAGATGA
GATCCAGTCCTGCAGGCTGC
60 114–118
20 MCW0069 26 GCACTCGAGAAAACTTCCTGCG
ATTGCTTCAGCAAGCATGGGAGGA
60 158–176