Table 2.
No. | Primer | Chromosomal location | Primer sequence (5′→3′), Forward and reverse | Annealing Temp. (°C) | Allele size range (bp) |
---|---|---|---|---|---|
1 | MCW0248 | 1 | GTTGTTCAAAAGAAGATGCATG TTGCATTAACTGGGCACTTTC |
60 | 205–225 |
2 | MCW0111 | 1 | GCTCCATGTGAAGTGGTTTA ATGTCCACTTGTCAATGATG |
60 | 96–120 |
3 | ADL0268 | 1 | CTCCACCCCTCTCAGAACTA CAACTTCCCATCTACCTACT |
60 | 102–116 |
4 | MCW0020 | 1 | TCTTCTTTGACATGAATTGGCA GCAAGGAAGATTTTGTACAAAATC |
60 | 179–185 |
5 | LEI0234 | 2 | ATGCATCAGATTGGTATTCAA CGTGGCTGTGAACAAATATG |
60 | 216–364 |
6 | MCW0206 | 2 | ACATCTAGAATTGACTGTTCAC CTTGACAGTGATGCATTAAATG |
60 | 221–249 |
7 | MCW0034 | 2 | TGCACGCACTTACATACTTAGAGA TGTCCTTCCAATTACATTCATGGG |
60 | 212–246 |
8 | MCW0103 | 3 | AACTGCGTTGAGAGTGAATGC TTTCCTAACTGGATGCTTCTG |
64 | 266–270 |
9 | LEI0166 | 3 | CTCCTGCCCTTAGCTACGCA TATCCCCTGGCTGGGAGTTT |
60 | 354–370 |
10 | MCW0295 | 4 | ATCACTACAGAACACCCTCTC TATGTATGCACGCAGATATCC |
60 | 88–106 |
11 | MCW0081 | 5 | GTTGCTGAGAGCCTGGTGCAG CCTGTATGTGGAATTACTTCTC |
60 | 112–135 |
12 | MCW0014 | 6 | TATTGGCTCTAGGAACTGTC GAAATGAAGGTAAGACTAGC |
58 | 164–182 |
13 | MCW0183 | 7 | ATCCCAGTGTCGAGTATCCGA TGAGATTTACTGGAGCCTGCC |
58 | 296–326 |
14 | ADL0278 | 8 | CCAGCAGTCTACCTTCCTAT TGTCATCCAAGAACAGTGTG |
60 | 114–126 |
15 | MCW0067 | 10 | GCACTACTGTGTGCTGCAGTTT GAGATGTAGTTGCCACATTCCGAC |
60 | 176–186 |
16 | MCW0104 | 13 | TAGCACAACTCAAGCTGTGAG AGACTTGCACAGCTGTGTACC |
60 | 190–234 |
17 | MCW0123 | 14 | CCACTAGAAAAGAACATCCTC GGCTGATGTAAGAAGGGATGA |
60 | 76–100 |
18 | MCW0330 | 17 | TGGACCTCATCAGTCTGACAG AATGTTCTCATAGAGTTCCTGC |
60 | 256–300 |
19 | MCW0165 | 23 | CAGACATGCATGCCCAGATGA GATCCAGTCCTGCAGGCTGC |
60 | 114–118 |
20 | MCW0069 | 26 | GCACTCGAGAAAACTTCCTGCG ATTGCTTCAGCAAGCATGGGAGGA |
60 | 158–176 |