Skip to main content
. 2018 Jul 24;46(19):10340–10352. doi: 10.1093/nar/gky666

Figure 4.

Figure 4.

Longer Pten 3′-UTR isoforms are more stable. (A) Representative northern blot with NMuMG cells showing the decay of each Pten 3′-UTR isoform upon actinomycin-D (Act-D) treatment. Cells were harvested at 0, 30, 60, 120, 150, 180, 240, 300 and 360 min post-treatment. Dimethyl sulfoxide (DMSO) was used to solubilize Act-D and served as a mock control. (B) Each Pten 3′-UTR isoform was quantified with the specific isoform qRT-PCR assay, Pten 300, Pten 3.3 and Pten 5.5/6.1. c-Myc mRNA was used as a positive control for the Actinomycin-D treatment and was also quantified by qRT-PCR. Total mRNA was normalized with Hprt, and mRNA half-life was determined by linear regression analysis. (C) The bar graph shows the half-life in minutes calculated for each Pten 3′-UTR isoform, 300-nt, 3.3, 5.5/6.1 kb and positive control c-Myc from the qRT-PCR analysis. Data shown are means ± standard deviation from four independent biological experiments. A two-tailed Student’s t-test was used (*P ≤ 0.05).