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Effect of cpzA3H mutations in the RNA recognition interface on the intracellular levels of cpzA3H. WT cpzA3H and mutants were transiently expressed in 293T cells in the absence of an inhibitor or in the presence of MG132 (8 μM), chloroquine (100 μM) or bafilomycin A1 (80 nM). The proteins were detected by western blotting with the anti-DYKDDDDK tag monoclonal antibody (Anti-FLAG) or anti-β-tubulin antibody (Anti-β-Tubulin).
