Figure 5.

Exogenous H2B E76K production enhances malignant transformation of cells. (A) Colony formation assay with NIH3T3 cells expressing H2B or H2B E76K. Representative images are shown. Three independent experiments were performed, and similar results were obtained. (B) Graphic representation of the experiments shown in panel A. The colony numbers of cells expressing H2B or H2B E76K are plotted. Means ± s.d. (n = 3) are shown. (C) Western blotting analyses. The production of exogenous H2B and H2B E76K was detected by western blotting, using the α-GFP polyclonal antibody (upper panel). In this system, GFP was simultaneously produced with H2B or H2B E76K, from a polycistronic gene. For loading controls, endogenous β-actin was detected by western blotting using the anti-β-actin antibody (lower panel). The full images of the western blots are shown in Supplementary Figures S13C and D.