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. 2018 Sep 20;46(19):10272–10285. doi: 10.1093/nar/gky815

Figure 4.

Figure 4.

Indels mediated by different Cpf1 nucleases on endogenous targets with TTTV PAM sequence in HEK293 cells. Indels mediated by different Cpf1 nucleases (blue bars: LbCpf1, orange bars: AsCpf1, green bars: FnCpf1 and yellow bars: MbCpf1) on 17 endogenous targets with TTTV PAM sequence. The nuclease vector along with the corresponding crRNA expressing vector were transfected into HEK293 cells and indels were analyzed 5 days post-transfection by deep sequencing. Three parallel transfections were made for each sample. Tukey-type notched boxplots by BoxPlotR (53): center lines show the medians; box limits indicate the 25th and 75th percentiles; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles; notches indicate the 95% confidence intervals for medians; crosses represent sample means; data points are plotted as open circles that correspond to the different targets tested. See also Supplementary Figures S7 and S8.