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Summary of PAM requirement of Lb-, As-, Mb- and FnCpf1 nucleases. Ratios of activities of the four nucleases averaged over targets in which the nucleotides have been varied systematically at one of the positions of the TTTN PAM sequence. Base preference at (A) -4; (B) -3; (C) -2; (D) -1 PAM positions. Red: thymidine, blue: cytosine, green: adenine, gray: guanine. See also Supplementary Figures S9–S12.
