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. 2018 Sep 22;46(19):10246–10261. doi: 10.1093/nar/gky854

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Loop dependent recognition of G-quadruplex structure by the RGG-box. (A) 2D ¹⁵N–¹H HSQC spectrum of the free RGG-box (black) and in complex with Tel6 at 1:6 protein to DNA molar ratio (red). No significant chemical shift perturbations were observed for this interaction. Tetrameric G-quadruplex form of Tel6 that is devoid of loops is shown as a cartoon. (B) 2D ¹⁵N–¹H HSQC spectrum of the free RGG-box (black) and in complex with Tel12 at 1:1 protein to DNA molar ratio (red). Dimeric G-quadruplex form of Tel12 consisting of two loops is shown as a cartoon. Specific chemical shift perturbations were observed for several residues (marked with green arrows). (C) A subset of residues of RGG-box that show specific chemical shift perturbation upon addition of Tel12 is shown. The RGG-box and Tel12 complex was in fast to intermediate exchange. Three steps of titration at different protein to DNA ratios are shown: black at 1:0, green at 1:0.5 and red at 1:1.