Table 1.
Restraints and structural statistics for Rok-C97-191 and Rok-C102-185/Seq1 complex
| Rok-C97–191 | Rok-C102–185/Seq1 | |
|---|---|---|
| Restrains for protein | ||
| NOE | 3078 | 2542 |
| Intra-residues | 858 | 687 |
| Inter-residues | 1046 | 822 |
| Sequential | 452 | 393 |
| Non-sequential | 594 | 429 |
| Ambiguous | 1174 | 1033 |
| Dihedral angle restraints | 110 | 110 |
| φ angle | 57 | 55 |
| ψ angle | 57 | 55 |
| Chirality restraints | 385 | 351 |
| ω angle | 103 | 92 |
| Chirality | 282 | 259 |
| Restraints for DNA | ||
| NOE | 983 | |
| Hydrogen bonds | 62 | |
| Sugar pucker | 36 | |
| Backbone dihedral angle | 244 | |
| Protein–DNA intermolecular NOE | 62 | |
| Distance restraints violations (>0.2 Å) | 0 | 0 |
| Dihedral angle restraints violations (>5°) | 0 | 0 |
| Average pairwise RMSD (Å) | ||
| Protein (all heavy atoms) | 1.04 ± 0.08 | 0.99 ± 0.19 |
| Protein (backbone heavy atoms) | 0.40 ± 0.10 | 0.44 ± 0.13 |
| DNA (all heavy atoms) | 0.69 ± 0.21 | |
| Complex (all heavy atoms) | 0.71 ± 0.19 | |
| Ramachandran plot | ||
| Most favored regions (%) | 92.8 | 88.5 |
| Additionally allowed regions (%) | 7.2 | 11.5 |
| Generously allowed regions (%) | 0.1 | 0 |
| Disallowed regions (%) | 0 | 0 |