Table 2.

Equilibrium dissociation constants of Rok-C^102–185, Rok-C^114–185 and its mutants binding to Seq1 DNA, measured using ITC. The uncertainties indicate the combined standard deviations of two measurements (N/D: not detectable)

	K d (μM)
Rok-C102–185	7.9 ± 1.3
Rok-C114–185	19.7 ± 2.7
R174A	89 ± 42
T156A	42.9 ± 7.1
T157A	15.0 ± 1.9
N154D	N/D
K171A	110 ± 35
K116A	42.9 ± 8.1
K136A	39.4 ± 6.4
K164A	30.9 ± 2.9