Table 2.
Proof-of-principle DNA assembly using BsaI, BpiI or Lgui-based MetClo systems
Plasmid | Vector | Inserts | Methylase | Enzyme | Size | Success rate | |||
---|---|---|---|---|---|---|---|---|---|
pMXP_test | pMXP_A4E | pMXK2_FragA | pMXK2_FragB | pMXK2_FragC | pMXK2_FragD | M.Osp807II | BsaI | 908 bp | 100% (8/8) |
pMYP_test | pMYP_A5E | pMYK2_FragA | pMYK2_FragB | pMYK2_FragC | pMYK2_FragD | M2.NmeMC58II | BpiI | 1152 bp | 100% (8/8) |
pMZP_test | pMZP_P6T | pMZK2_FragA | pMZK2_FragB | pMZK2_FragC | pMZK2_FragD | M.XmnI | LguI | 1149 bp | 100% (8/8) |