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. 2018 Oct 18;10(10):570. doi: 10.3390/v10100570

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Quantitative real-time PCR (qRT-PCR) validation of differentially expressed genes from RNA sequencing. qRT-PCR analyses were normalized using GAPDH as an internal control gene. TMV: TMV resistance protein; PRP: Pathogenesis-related protein 1; NAC: NAC transcription factor 29; DZF: Dof zinc finger protein; MLP: MLP-like protein 423; GTE: GTE7-transcriptional factor; BRP: Brassinosteroid-regulated protein bru1; AP2: AP2-like ethylene-responsive transcription factor; PNM: Phosphomethylethanolamine N-methyltransferase; AUX: Auxin efflux carrier component; SFH: S-formylglutathione hydrolase; BEL: Homeobox protein BEL1. qRT-PCR analyses were normalized using GAPDH as an internal control gene. The fold change of each gene was calculated by the 2^−ΔΔCt method.