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. 2018 Oct 30;9(5):e01831-18. doi: 10.1128/mBio.01831-18

TABLE 2.

Frequencies of cells harboring viral genomes in C57BL/6 mice

Virusa Route of
infectionb 		Mouse Organc No. of
dpi		 Total no.
of cells
harvestedd 		Frequency of
genome-positive
cellse 		Total no. of cells
positive for
latent virusf
ORF46.stop i.n. WT Spleen 16 1.8 × 108 BLDg ,h BLDh
ORF46.stop MR i.n. WT Spleen 16 3.1 × 108 302g 1.0 × 106
ORF46.CM i.n. WT Spleen 16 2.4 × 108 578 4.1 × 105
ORF46.CM MR i.n. WT Spleen 16 2.2 × 108 328 6.6 × 105
ORF54.CM i.n. WT Spleen 16 1.1 × 108 4,197 2.5 × 104
ORF54.CM MR i.n. WT Spleen 16 1.4 × 108 437 3.1 × 105
46.CM/54.CM i.n. WT Spleen 16 9.8 × 107 24,346 4.0 × 103
46.CM/54.CM MR i.n. WT Spleen 16 1.4 × 108 266 5.2 × 105
ORF46.CM i.n. WT Spleen 42 1.6 × 108 7,837 2.0 × 104
ORF46.CM MR i.n. WT Spleen 42 1.4 × 108 2,458 5.8 × 104
ORF54.CM i.n. WT Spleen 42 1.1 × 108 2,258g 4.6 × 104
ORF54.CM MR i.n. WT Spleen 42 1.3 × 108 1,840g 7.0 × 104
46.CM/54.CM i.n. WT Spleen 42 1.5 × 108 18,798 7.8 × 103
46.CM/54.CM MR i.n. WT Spleen 42 1.5 × 108 6,801 2.1 × 104
ORF46.CM i.p. WT Spleen 16 2.7 × 108 154 1.8 × 106
ORF46.CM MR i.p. WT Spleen 16 2.9 × 108 248 1.2 × 106
ORF54.CM i.p. WT Spleen 16 2.3 × 108 393 5.7 × 105
ORF54.CM MR i.p. WT Spleen 16 2.3 × 108 226 1.0 × 106
46.CM/54.CM i.p. WT Spleen 16 2.4 × 108 351 6.8 × 105
46.CM/54.CM MR i.p. WT Spleen 16 2.3 × 108 294 7.8 × 105
ORF46.CM i.p. WT PECs 16 1.9 × 107 203 9.6 × 104
ORF46.CM MR i.p. WT PECs 16 3.4 × 107 131 2.6 × 105
ORF54.CM i.p. WT PECs 16 1.5 × 107 146 1.0 × 105
ORF54.CM MR i.p. WT PECs 16 1.2 × 107 86 1.4 × 105
46.CM/54.CM i.p. WT PECs 16 8.9 × 106 184 4.9 × 104
46.CM/54.CM MR i.p. WT PECs 16 1.2 × 107 85 1.4 × 105
ORF46.stop i.p. UNG−/− Spleen 42 7.2 × 107 24,804i 2.9 × 103
i.p. WT Spleen 42 6.5 × 107 22,078i 2.9 × 103
ORF46.stop MR i.p. UNG−/− Spleen 42 7.0 × 107 13,445i 5.2 × 103
i.p. WT Spleen 42 5.7 × 107 13,060i 4.4 × 103
ORF46.CM i.p. WT Spleen 42 1.5 × 108 4,438 3.5 × 104
ORF46.CM MR i.p. WT Spleen 42 1.9 × 108 3,219 6.0 × 104
46.CM/54.CM i.p. WT Spleen 42 1.5 × 108 2,579 5.8 × 104
46.CM/54.CM MR i.p. WT Spleen 42 1.3 × 108 1,730 7.6 × 104
a

Infection with recombinant MHV68 viruses.

b

i.n., intranasal; i.p., intraperitoneal.

c

Organ harvested for limiting dilution analysis.

d

The total number of cells harvested per mouse was determined by the mean of pooled cells from three to six mice per experiment unless indicated elsewhere.

e

Data represent frequencies of the indicated cells (1 in x) that harbored viral genome based on Poisson distribution analysis. The frequency data were determined from the mean of two to five independent experiments with cells from the indicated organs; organs were pooled from three to six mice per experiment unless indicated elsewhere.

f

The total number of genome-positive cells per mouse was extrapolated using the frequency value generated from the limiting dilution analysis together with the total number of splenocytes or PECs harvested.

g

The frequency data were determined from one experiment with splenocytes pooled from four to five mice.

h

BLD, below the limit of detection.

i

The frequency data were determined from the mean of four to five mice with the splenocytes harvested.