FIG 3.
PPAD stabilizes gingipains and modulates the levels of phagocytosis-related proteins. (a) Relative (Rel.) levels of gingipains (RgpA/RgpB) in infected neutrophils. (b) Time course of histone H3 degradation by P. gingivalis proteases in the presence or absence of PPAD, as determined by Western blotting (SN, culture supernatant). (c and d) Quantification of significant changes in the amounts of phagocytosis-related proteins (c) and the antimicrobial histone H2B (d) in infected neutrophils, as approximated by mass spectrometry. (a, c, and d) Data are means of three replicates of one neutrophil donor. **, P < 0.01, two-tailed unpaired Student’s t tests. Data are presented as mean values ± SD. *, P < 0.05, Fisher’s exact test. Green and red arrows indicate up- or downregulation of >10% of the respective protein in W83-infected neutrophils. Norm., normalized.