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Confocal scanning laser microscopy (CSLM) images of H. pylori SS1 biofilm. Shown are representative CSLM images of 3-day-old SS1 biofilms grown in BB2 and stained with (A) FM 1–43 to stain total bacterial cells, (B) SYPRO RUBY to stain extracellular proteins, (C) BOBO-3 to stain extracellular DNA, and (D to F) live-dead staining with live cells represented by the green fluorescent SYTO 9 and dead/damaged cells represented by the red fluorescent propidium. Scale bar = 30 µm.
