FIG 3.

Effect of enzymatic treatments on preformed biofilms. H. pylori SS1 was allowed to form biofilms for 3 days in BB2. The medium was then removed and replaced with either fresh medium or medium containing DNase I or proteinase K. Cells were reincubated for 24 h and then analyzed for the remaining biofilm using the crystal violet assay. The data shown here represent the percentage of remaining biofilm compared to the untreated control. Experiments were performed three times independently with at least 8 technical replicates for each. Statistical analysis was performed using ANOVA (*, P < 0.01 compared to the untreated control).