FIG 8.

H. pylori G27 biofilm contains structurally important flagella. (A) Scanning electron microscope (SEM) images of wild-type G27 H. pylori biofilms. Arrows indicate flagella. (B) Quantification of biofilm formation by the H. pylori G27 wild type (WT), the nonmotile flagellated motB mutant, the nonmotile fliA mutant that is reported to have either truncated flagella or no flagella, and the aflagellated and nonmotile flgS mutant. Biofilms were evaluated using the crystal violet assay. Experiments were performed 2 times independently with at least 6 technical replicates for each. Statistical analysis was performed using ANOVA (**, P < 0.01; and *, P < 0.05).