FIG 5.

Either CatR or YodB facilitates Fur binding at the promoter site of catDE. Fur occupancy was evaluated by chromatin immunoprecipitation (ChIP) using anti-FLAG antibodies. Coimmunoprecipitated DNA was quantified by qPCR. DNA enrichment was calculated based on the input DNA (1% of total DNA used for each ChIP experiment). The data are presented as the fold enrichment of Fur occupancy at the promoter sites of catDE (A) and dhbA (B) (mean ± SD; n = 3). Significant differences between WT and mutant strains are indicated: **, P < 0.01. No significant DNA enrichment was observed for gyrA, which serves as a negative control.