FIG 3.

Potency and selectivity for inhibition of B. mandrillaris viability by nitroxoline, pentamidine isethionate, miltefosine, and azithromycin. (A) Dose-response curves show the effect of nitroxoline (red), pentamidine isethionate (black), miltefosine (blue), and azithromycin (gray) on the viability of B. mandrillaris trophozoite populations following 72 h of treatment. Data points represent means and standard errors of results from at least three independent biological replicates. Nitroxoline was the most potent inhibitor of B. mandrillaris viability, with an IC₅₀ of 2.84 µM. (B) Heat map showing the Log₁₀ selectivity index (human cell CC₅₀/B. mandrillaris IC₅₀) for nitroxoline, pentamidine, isethionate, miltefosine, and azithromycin calculated from the ratio of human cell CC₅₀ to B. mandrillaris IC₅₀. Nitroxoline exhibited the greatest mean Log₁₀ selectivity index at 0.832 and was the only drug with a positive Log₁₀ selectivity index (comparing B. mandrillaris inhibition to the results from all cell lines tested).