Table 1.
Representative patterns of HDR and NHEJ editing with pDonor-sg or ssODN at different loci
| HDR and NHEJ at EEF1A1 with pDonor-sg | |||
| Total reads = 33,041 | Type | Indel (bp) | Read % |
| ATACAACTGAACAGTAC|TTTGGG | WT | 0 | 37.9% |
| ATACAACTGAACAGgtttaaacgcgtGGG | HDR | −6, +12 | 49.0% |
| ATACAACTGAACAGTACcTTTGGG | NHEJ | +1 | 0.8% |
| ATACAACTGAACAGTACaTTTGGG | NHEJ | +1 | 0.8% |
| ATACAACTGAACA———G | NHEJ | −9 | 0.7% |
| ATACAACTGAACAGTAC-TTGGG | NHEJ | −1 | 0.6% |
| ATACAACTGAACAGTACttTTTGGG | NHEJ | +2 | 0.5% |
| ATACAACTGAACAGTAC–TGGG | NHEJ | −2 | 0.5% |
| ATACAACTGAACAGTAC—GGG | NHEJ | −3 | 0.3% |
| ATACAACTGAACAGTA-TTTGGG | NHEJ | −1 | 0.3% |
| ATACAACTGAACAG—-TTGGG | NHEJ | −4 | 0.3% |
| ATACAACTGAACAGTACtTTTGGG | NHEJ | +1 | 0.3% |
| HDR and NHEJ at GAPDH with pDonor-sg | |||
| Total reads = 139,341 | Type | Indel (bp) | Read % |
| GACTGGCTCTTAAAAAG|TGCAGG | WT | 0 | 42.3% |
| GACTGGCTCTTgtttaaacgcgtTGCAGG | HDR | −6, +12 | 15.8% |
| GACTGGCTCTTAA—-TGCAGG | NHEJ | −4 | 3.1% |
| GACTGGC—————- | NHEJ | −23 | 2.8% |
| GACTGGCTCTTA—–TGCAGG | NHEJ | −5 | 1.9% |
| GACTGGCTCTTAAAA—–AGG | NHEJ | −5 | 1.7% |
| GACTGGCTCTTAAAAA–GCAGG | NHEJ | -2 | 1.7% |
| GACTGGCTCTTAAAA–TGCAGG | NHEJ | −2 | 1.6% |
| GACTGGCTCTTAAAAA-TGCAGG | NHEJ | −1 | 0.8% |
| GACTGGCTCTTAAAAA——G | NHEJ | −6 | 0.8% |
| GACTGGCTCTTAAAAAGtTGCAGG | NHEJ | +1 | 0.7% |
| GACTGGCTC————– | NHEJ | −23 | 0.7% |
| HDR and NHEJ at AAVS1 with ssODN donor | |||
| Total reads = 33,631 | Type | Indel (bp) | Read % |
| GGGGCCACTAGGGACAG|GATTGG | WT | 0 | 74.9% |
| GGGGCCACTAGGGACAGacgcgtGATTGG | HDR | +6 | 1.0% |
| GGGGCCACTAGGGACA-GATTGG | NHEJ | −1 | 6.4% |
| GGGGCCACTAGGG—–ATTGG | NHEJ | −5 | 2.8% |
| GGGGCCACTAGGGACAG—— | NHEJ | −12 | 2.1% |
| GGGGCCACTAGGGAC-GGATTGG | NHEJ | −1 | 1.4% |
| GGGGCCACTAGGGAC–GATTGG | NHEJ | −2 | 0.9% |
| GGGGCCACTAGGGACAGgGATTGG | NHEJ | +1 | 0.8% |
| GGGGCCACTA———–GG | NHEJ | −11 | 0.5% |
| GGGGCCACTAG———-GG | NHEJ | −10 | 0.4% |
| GGGGCCACTAGGGACAGcagGATTGG | NHEJ | +3 | 0.4% |
| GGGGCCACTAGGGA–GGATTGG | NHEJ | −2 | 0.3% |
| HDR and NHEJ at CD326 with ssODN donor | |||
| Total reads = 57,018 | Type | Indel (bp) | Read % |
| GTTCGGGCTTCTGCTTG|CCGCGG | WT | 0 | 46.7% |
| GTTCGGGCTTCTGCTTGacgcgtCCGCGG | HDR | +6 | 7.2% |
| GTTCGGGCTTCTGCTT-CCGCGG | NHEJ | −1 | 11.0% |
| GTTCGGGCTTCTGCTTgGCCGCGG | NHEJ | +1 | 3.1% |
| GTTCGGGCTTCTGCTT——- | NHEJ | −19 | 1.3% |
| GTTCGGGCTTCTGCTTG-CGCGG | NHEJ | −1 | 1.2% |
| GTTCGGGCTTCTG—-CCGCGG | NHEJ | −4 | 1.1% |
| GTTCGGGCTT——-CCGCGG | NHEJ | −7 | 1.1% |
| GTTCGGGCTTCTGCT–CCGCGG | NHEJ | −2 | 0.9% |
| GTTCG—————-GG | NHEJ | −16 | 0.8% |
| GTTCGGGCTTCTGC——— | NHEJ | −25 | 0.8% |
| GTTCGGGCTTCTGCTT——G | NHEJ | −6 | 0.8% |